Complete Media - Animal tissue culture

The term complete medium implies a medium that has had all its constituents and supplements added and is sufficient for the use specified. It is usually made up of a defined medium component, some of the constituents, such as glutamine, may be added just before use, and various supplements, such as serum, growth factors, or hormones.

Defined media range in complexity from the relatively simple Eagle’s MEM, which contains essential amino acids, vitamins, and salts, to complex media such as medium 199 (M199), CMRL 1066, MB 752/1, RPMI 1640, and F12 and a wide range of serum-free formulations. The complex media contain a larger number of different amino acids, including nonessential amino acids and additional vitamins, and are often supplemented with extra metabolites (e.g., nucleosides, tri carboxylic acid cycle intermediates, and lipids) and minerals. Nutrient concentrations are, on the whole, low in F12 (which was optimized by cloning) and high in Dulbecco’s modification of Eagle’s MEM (DMEM), optimized at higher cell densities for viral propagation. Barnes and Sato [in 1980] used a 1:1 mixture of DMEM and F12 as the basis for their serum-free formulations to combine the richness of F12 and the higher nutrient concentration of DMEM. Although not always entirely rational, this combination has provided an empirical formula that is suitable as a basic medium for supplementation with special additives for many different cell types.

 

--------------------------------------------------

 

Did you read these ?

Development of media for animal tissue culture

Use of Antibiotics in Animal Tissue Culture for maintaining sterility

Balanced Salt Solution in Animal Tissue Culture

Animal Biotechnology - Tissue Culture and Cell Culture 

Maintenance of Sterility in Animal Tissue Culture Labs